0
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 22.6 | 68.7 | 116 | 21.7 | 70.4 | 119 |
| Standard Deviation | 1.37 | 2.55 | 2.54 | 2.2 | 6 | 7.3 |
| CV% | 6 | 3.7 | 2.2 | 10.3 | 8.6 | 6.1 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 18.5 | 56.9 | 96.1 | 17 | 51.8 | 89.2 |
| Standard Deviation | 0.89 | 1.74 | 2.73 | 1.3 | 3.5 | 6.9 |
| CV% | 4.8 | 3.1 | 2.8 | 7.4 | 6.8 | 7.7 |
The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 104 | 86-119 |
| Cell Lysis Buffer (n=1) | 91 | 87-94 |
| EDTA Plasma (n=4) | 105 | 92-120 |
| Heparin Plasma (n=4) | 98 | 85-111 |
| Saliva (n=4) | 97 | 80-117 |
| Serum (n=4) | 103 | 97-114 |
| Urine (n=6) | 100 | 91-119 |
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
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