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Format
96-well strip plate
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Assay Length
< 1 hour
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Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Citrate Plasma (25 uL), Urine (10 uL)
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Sensitivity
0.78 umol/L
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Assay Range
3.1 - 200 umol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
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Specificity
Measures Total Nitric Oxide, Nitrite and Nitrate levels in various samples
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Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
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Interference
Interference observed with other substances.
Control Available
QC135, Parameter Immunoassay Control Set 904 for Nitrite -
QC134, Parameter Immunoassay Control Set 849 for Nitrate -
Product Summary
The Parameter Total Nitric Oxide kit has two assay options. Endogenous nitrite is measured in the first option. In the second option, nitrate is converted to nitrite using nitrate reductase and total nitrite is measured. To obtain the nitrate concentration, endogenous nitrite is subtracted from the total nitrite value.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 30 | 77.2 | 138 | 31.5 | 79.9 | 138.7 |
| Standard Deviation | 0.76 | 1.1 | 2.2 | 1.46 | 2.91 | 4.78 |
| CV% | 2.5 | 1.4 | 1.6 | 4.6 | 3.6 | 3.4 |
Recovery
The recovery of the nitrate standard spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (Nitrite Assay) (n=10) | 107 | 91-115 |
| Plasma (Nitrate Assay) (n=25) | 98 | 87-110 |
| Plasma (Nitrite Assay) (n=20) | 104 | 92-117 |
| Serum (Nitrate Assay) (n=10) | 95 | 89-101 |
| Serum (Nitrite Assay) (n=10) | 109 | 98-118 |
| Urine (Nitrite Assay) (n=10) | 101 | 87-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of the nitrate standard were serially diluted with the Reaction Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
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Alternate Names
Nitric Oxide;
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