-
Assay Length
4.5 hours
-
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
-
Sensitivity
1.5 pg/mL
-
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates)
-
Specificity
Natural and recombinant mouse IL-13
-
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
-
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-13 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 levels in cell culture supernates. It contains E. coli-expressed recombinant mouse IL-13 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 accurately. Results obtained using natural mouse IL-13 showed dose curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 33.9 | 100 | 432 | 31.6 | 93.4 | 405 |
| Standard Deviation | 1.1 | 2.7 | 11.2 | 2.5 | 7.2 | 17.7 |
| CV% | 3.2 | 2.7 | 2.6 | 7.9 | 7.7 | 4.4 |
Recovery
The recovery of mouse IL-13 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=13) | 99 | 89-119 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with mouse IL-13 were diluted with Calibrator Diluent and assayed.
Preparation and Storage
Background: IL-13
IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
新浪微博
腾讯微博
QQ空间
客服
