Mouse IL-12 p70 Quantikine SixPak (6 Plates) 1 PK

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Qiagen 凯杰 Biosharp Omega

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Mouse IL-12 p70 Quantikine SixPak (6 Plates) 1 PK

市场价：: 24786.00

促销价：: ¥24786.00

货号：SM1270

品牌：R&D Systems

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计7-14工作日到货(以R&D Systems官网货期为准))

工作时间

周一至周五：9:00-18:00

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0771-3293894

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商品介绍
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Assay Type

Solid Phase Sandwich ELISA
Format

96-well strip plate
Assay Length

4.5 hours
Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity

2.5 pg/mL
Assay Range

7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity

Natural and recombinant mouse IL-12 p70
Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	36	44	36
Mean	28	68	193	20	56	230
Standard Deviation	2.5	6.5	19.4	2.3	6.2	20
CV%	8.9	9.6	10.1	11.5	11.1	8.7

Recovery

The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=7)	101	95-106
Serum (n=7)	97	88-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-12 p70 in each matrix were diluted with Calibrator Diluent and assayed.

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name

Interleukin 12 p70

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat);

Alternate Names

CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35;