Mouse IL-12/IL-23 p40 Allele-specific Quantikine SixPak 1 PK

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Mouse IL-12/IL-23 p40 Allele-specific Quantikine SixPak 1 PK

市场价：: 24786.00

促销价：: ¥24786.00

货号：SM1240

品牌：R&D Systems

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计7-14工作日到货(以R&D Systems官网货期为准))

工作时间

周一至周五：9:00-18:00

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0771-3293894

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商品介绍
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Assay Type

Solid Phase Sandwich ELISA
Format

96-well strip plate
Assay Length

4.5 hours
Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity

4 pg/mL
Assay Range

11.7 - 750 pg/mL (Cell Culture Supernates, Serum)
Specificity

Natural and recombinant mouse IL-12/IL-23 p40
Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 p40 homodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-12/IL-23 p40. Results obtained using natural mouse IL-12/IL-23 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12/IL-23 p40.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	42.2	161	588	41.9	151	581
Standard Deviation	0.81	1.7	9.6	4.1	10.3	24
CV%	1.9	1.1	1.6	9.8	6.8	4.1

Recovery

The recovery of mouse IL-12/IL-23 p40 spiked to levels in samples throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=11)	100	81-116
Serum (n=7)	104	85-115

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-12/IL-23 p40 in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12/IL-23 p40

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.