* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).
For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
This kit is validated for human serum, EDTA plasma, and heparin plasma samples. All samples have a recommended starting dilution of 1:2.
Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
GM-CSF | 0.13 | 1,600 | 29 | |
IFN-gamma | 0.25 | 22,400 | 30 | |
IL-1 beta/IL-1F2 | 0.18 | 1,200 | 20 | |
IL-2 | 0.28 | 2,500 | 19 | |
IL-4 | 2.54 | 7,200 | 21 | |
IL-5 | 0.12 | 1,400 | 22 | |
IL-6 | 0.31 | 3,900 | 25 | |
CXCL8/IL-8 | 0.07 | 3,200 | 26 | |
IL-10 | 0.24 | 1,900 | 27 | |
IL-12 p70 | 2.96 | 28,300 | 28 | |
TNF-alpha | 0.54 | 3,100 | 12 | |
VEGF | 1.35 | 2,300 | 13 | |
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
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