The Cultrex® Directed In Vivo Angiogenesis Assay (DIVAA™) is designed to assess angiogenesis in an in vivo system. The DIVAA Starter Kit is designed to give new users practical experience using this assay system and is ideal for optimizing assay conditions. During the course of the assay, implant grade silicone cylinders closed at one end, called angioreactors, are filled with basement membrane extract (BME) premixed with or without angiogenesis modulating factors. These angioreactors are then implanted subcutaneously in the dorsal flanks of nude mice. If filled with angiogenic factors, vascular endothelial cells migrate into, and proliferate in the BME to form vessels in the angioreactor. As early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic factors. Each kit contains 48 angioreactors and enough growth factor to induce angiogenesis in all of the provided angioreactors. The DIVAA Starter Kit also supplies an autoclavable and reusable AngioRack™ to hold the angioreactors while they are being prepared for implantation.
Evaluation of Angiogenesis Activation using DIVAA. DIVAA was used to assess angiogenesis in nude mice stimulated by either fibroblast growth factor-2 alone (FGF-2, 100 ng) or in combination with vascular endothelial growth factor (FGF-2, 37 ng; VEGF, 12.5 ng). Angiogenesis was measured as the amount of FITC-Lectin detected 9 days post-implantation. Data is shown as the amount of fluorescence detected in treated samples (FGF-2, FGF-2/VEGF) versus the negative control (Untreated). |
Cultrex and PathClear are registered trademarks of Trevigen, Inc.
DIVAA, AngioRack, and CellSperse are trademarks of Trevigen, Inc.
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