The Cultrex® Directed In Vivo Angiogenesis Assay (DIVAA™) is designed to assess angiogenesis in an in vivo system. During the course of the assay, implant grade silicone cylinders closed at one end, called angioreactors, are filled with 20 μL of Trevigen's basement membrane extract (BME) premixed with or without angiogenesis modulating factors. These angioreactors are then implanted subcutaneously in the dorsal flanks of nude mice. If filled with angiogenic factors, vascular endothelial cells migrate into, and proliferate in the BME to form vessels in the angioreactor. As early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic factors. Each kit contains 48 angioreactors and enough growth factor to induce angiogenesis in all 48 experiments.
Features
- Utilizes a standardized platform for reproducible and quantifiable results
- Results seen in as few as 9 days post-implantation
- Up to four angioreactors can be implanted in each mouse, allowing for greater statistical power
- Uses a fraction of materials conserving BME and test compounds
- Averts assay errors caused by absorption of BME by the mouse
Kit Contents
- Angioreactors
- Growth Factor Reduced PathClear® BME
- 10X Wash Buffer
- CellSperse™
- 200X FITC-Lectin
- 25X FITC-Lectin Diluent
- Heparin Solution
- FGF-2 (1.8 µg)/VEGF (600 ng)
Cultrex and PathClear are registered trademarks of Trevigen, Inc.
DIVAA, AngioRack, and CellSperse are trademarks of Trevigen, Inc.
Preparation and Storage
Background: Angiogenesis Assay Kits
Angiogenesis is the tightly regulated process by which new blood vessels are formed from the existing vasculature. This process is physiologically important for development and wound healing, and is also a common driver in multiple diseases including rheumatoid arthritis, atherosclerosis, macular degeneration, and cancer. Angiogenesis occurs in response to a variety of molecular cues. Generally, the angiogenic process includes endothelial cell proliferation, chemotactic endothelial cell migration through the extracellular matrix barrier, and the formation of capillary tubes. Physiological and pathological angiogenesis utilize many of the same cellular processes and molecular signaling networks, however the structures that form during pathological angiogenesis are often functionally abnormal.
-
Alternate Names:
Angiogenesis Assay Kits
新浪微博
腾讯微博
QQ空间
客服
