The Cultrex® Directed In Vivo Angiogenesis Assay (DIVAA™) is designed to assess angiogenesis in an in vivo system. During the course of the assay, implant grade silicone cylinders closed at one end, called angioreactors, are filled with basement membrane extract (BME) premixed with or without angiogenesis modulating factors. These angioreactors are then implanted subcutaneously in the dorsal flanks of nude mice. If filled with angiogenic factors, vascular endothelial cells migrate into, and proliferate in the BME to form vessels in the angioreactor. As early as nine days post-implantation, there are enough cells to determine an effective dose response to angiogenic factors. Each kit contains 48 angioreactors and enough growth factor for eight positive controls.
Evaluation of Angiogenesis Activation using DIVAA. DIVAA was used to assess angiogenesis in nude mice stimulated by either fibroblast growth factor-2 alone (FGF-2, 100 ng) or in combination with vascular endothelial growth factor (FGF-2 (300 ng)/VEGF (100 ng). Angiogenesis was measured as the amount of FITC-Lectin detected 9 days post-implantation. Data is shown as the amount of fluorescence detected in treated samples (FGF-2, FGF-2/VEGF) versus the negative control (Untreated). |
Cultrex and PathClear are registered trademarks of Trevigen, Inc.
DIVAA, AngioRack, and CellSperse are trademarks of Trevigen, Inc.
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