The PARP Universal Colorimetric Assay (Catalog # 4677-096-K) measures Poly (ADP-ribose) Polymerase (PARP) in cells and tissues by detecting the incorporation of biotinylated Poly (ADP-ribose) (PAR) onto histone proteins in a 96-stripwell microplate format. This assay is useful for testing whether DNA is damaged, if damaged DNA is from non-apoptotic cells, or the effectiveness of PARP inhibitors.
Features
- Colorimetric, non-radioactive format
- Amenable to high-throughput
- Highly sensitive: capable of measuring 0.01 Units of PARP/well
Kit Contents
- Histone-coated 96-stripwell Microplate
- PARP Enzyme-High Specific Activity
- PARP Buffer
- PARP Cocktaila
- 3-aminobenzamideb
- Streptavidin-HRP & Diluent
- TACS-Sapphire™
- Activated DNA
Data Example
Inhibition of PARP Activity by 3-aminobenzamide
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| Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit; B). The standard curve is used to translate absorbance values to activity units of PARP. |
aPARP Cocktail contains biotinylated NAD
b3-aminobenzamide is a PARP inhibitor
TACS-Sapphire is a trademark of Trevigen, Inc.
Preparation and Storage
Background: PARP
PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.
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Long Name:
Poly [ADP-ribose] Polymerase
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Entrez Gene IDs:
142 (Human); 11545 (Mouse)
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Alternate Names:
ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADPRT 1; ADPRT; ADPRTADP-ribosyltransferase NAD(+); EC 2.4.2; EC 2.4.2.30; NAD(+) ADP-ribosyltransferase 1; PARP apoptosis; PARP; PARP1; PARP-1; PARPADPRT1; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; PPOLpADPRT-1
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