* Sold separately (see Other Reagents Required, below)
** Following an overnight incubation with capture antibody, 2 hour blocking step, and cell stimulation.
*** When using the protocol provided.

PRODUCT SUMMARY
Complete are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.

PRODUCT FEATURES

• An economical alternative to ELISpot Kits
• Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
• Generic development protocols provide direction to start an optimization protocol
• Customize the assay to your specific needs

MODULE CONTENTS

• Capture Antibody
• Biotinylated-conjugated Detection Antibody

OTHER REAGENTS REQUIRED

• ELISpot Blue Color Module or equivalent (R&D Systems, Catalog # )
• PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered
• Wash Buffer - 0.05% Tween^® 20 in PBS
• Blocking Buffer - 1% BSA, 5% Sucrose in PBS
• Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
• 2 °C – 8 °C refrigerator
• 37 °C CO₂ incubator
• Positive Control - Use Recombinant or cells known to secrete
• 96-well plates - Nitrocellulose-bottom plates, PVDF-bottom Immunospot^® plates, or flat-bottom polystyrene Immulon^® ELISA plates
• Multi-channel pipette, squirt bottle, manifold dispenser, or automated microplate washer
• Dissection microscope or an automated ELISpot Reader
• Deionized H₂O

Trademarks and registered trademarks are the property of their respective companies.

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.