Select analytes you would like to measure from the list below. Please note that analytes with the same bead region are not compatible in the same assay and when selected will populate a new assay after clicking the "continue" button.
** Initial Starting Dilutions
Analyte | Data Sheet | Bead Region | Standard Curve (pg/mL) | Cell Culture Supernates | Serum | Plasma |
---|---|---|---|---|---|---|
Adiponectin | 46 | 466-340,000 | None | 1:100 | 1:100 | |
C-Reactive Protein | 48 | 20-14,800 | N/R | 1:100 | 1:100 | |
CCL2/MCP-1 | 33 | 3-2,100 | None | 1:4 | 1:4 | |
Factor D/Adipsin | 47 | 126-91,500 | None | 1:100 | 1:100 | |
IL-10 | 29 | 4-3,200 | None | 1:4 | 1:4 | |
IL-6 | 27 | 7-4,950 | None | 1:4 | 1:4 | |
Leptin | 45 | 50-36,500 | None | 1:4 | 1:4 | |
Resistin | 56 | 46-33,200 | None | 1:4 | 1:4 | |
Serpin E1 | 26 | 14-10,300 | N/R | 1:100 | 1:100 | |
TNF-alpha | 37 | 6-4,500 | None | 1:4 | 1:4 |
* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).
For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
---|---|---|---|---|
Adiponectin/Acrp30 | 6.4 | 340,000 | 46 | |
C-Reactive Protein | 1.4 | 14,800 | 48 | |
CCL2/MCP-1 | 0.16 | 2,100 | 33 | |
Complement Factor D/Adipsin | 1.8 | 91,500 | 47 | |
IL-6 | 0.36 | 4,950 | 27 | |
IL-10 | 0.13 | 3,200 | 29 | |
Leptin | 7.69 | 36,500 | 45 | |
Resistin | 0.85 | 33,200 | 56 | |
Serpin E1/PAI-1 | 0.2 | 10,300 | 26 | |
TNF-alpha | 0.6 | 4,500 | 36 | |
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Analyte | Cell Culture Supernate | Serum | EDTA Plasma | Heparin Plasma |
---|---|---|---|---|
Adiponectin/Acrp30 | None | 1:100 | 1:100 | 1:100 |
C-Reactive Protein | None | 1:100 | 1:100 | 1:100 |
CCL2/MCP-1 | None | 1:4 | 1:4 | 1:4 |
Complement Factor D/Adipsin | None | 1:100 | 1:100 | 1:100 |
IL-6 | None | 1:4 | 1:4 | 1:4 |
IL-10 | None | 1:4 | 1:4 | 1:4 |
Leptin | None | 1:4 | 1:4 | 1:4 |
Resistin | None | 1:4 | 1:4 | 1:4 |
Serpin E1/PAI-1 | None | 1:100 | 1:100 | 1:100 |
TNF-alpha | None | 1:4 | 1:4 | 1:4 |
Magnetic Luminex High Performance Assay multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Assays for the Luminex platform are offered as or . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. .
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