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* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).
For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
| Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
| EMMPRIN | 5.6 | 25,700 | 30 | |
| MMP-1 | 1.1 | 18,300 | 20 | |
| MMP-2 | 12.6 | 63,100 | 19 | |
| MMP-3 | 7.3 | 25,700 | 21 | |
| MMP-7 | 6.6 | 55,850 | 22 | |
| MMP-8 | 16.6 | 59,700 | 25 | |
| MMP-9 | 13.7 | 73,100 | 26 | |
| MMP-10 | 3.2 | 31,000 | 27 | |
| MMP-12 | 0.7 | 6,700 | 28 | |
| MMP-13 | 63.5 | 324,000 | 29 | |
| *A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. | ||||
| Analyte | Cell Culture Supernate | Serum | Heparin Plasma | Platelet-poor Heparin Plasma | Saliva | Urine |
| EMMPRIN | 1:5 | 1:5* | 1:5* | 1:5* | 1:40 | 1:10 |
| MMP-1 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-2 | N/R | 1:50 | 1:50 | 1:50 | 1:40 | 1:10 |
| MMP-3 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-7 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-8 | 1:5 | 1:50 | 1:50 | 1:50 | 1:40 | 1:10 |
| MMP-9** | 1:5 | 1:50 | N/R | 1:50 | 1:40 | 1:10 |
| MMP-10 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-12 | 1:5 | 1:50 | 1:50 | 1:50 | 1:40 | 1:10 |
| MMP-13 | 1:5 | 1:5 | N/R | N/R | 1:40 | 1:10 |
| N/R=This sample type is not recommended for use in this assay *When assaying serum and plasma samples, EMMPRIN cannot be multiplexed with MMP-7, MMP-8, MMP-10, MMP-12, or MMP-13. **MMP-9 is present in platelet granules and released upon platelet activation. Therefore, to measure circulating levels of this factor, platelet-free plasma should be collected for measurement. | ||||||
Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
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