For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
This kit is validated for human cell culture supernates, serum, EDTA plasma, and heparin plasma samples. All serum and plasma samples have a recommended starting dilution of 1:2. Cell culture supernate samples do not require dilution.
Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
GM-CSF | 0.368 | 6,550 | 29 | |
IFN-gamma | 0.25 | 5,800 | 30 | |
IL-1 beta/IL-1F2 | 0.146 | 4,240 | 20 | |
IL-2 | 0.386 | 4,850 | 19 | |
IL-5 | 0.132 | 2,100 | 22 | |
IL-6 | 0.135 | 2,650 | 25 | |
IL-7 | 0.140 | 3,390 | 33 | |
IL-13 | 2.01 | 25,600 | 34 | |
IL-15 | 0.167 | 2,420 | 35 | |
IL-17A | 0.349 | 10,370 | 36 | |
IL-17F | 4.54 | 36,750 | 37 | |
IL-22 | 0.529 | 6,700 | 38 | |
IL-23 | 9.52 | 70,000 | 39 | |
IL-31 | 0.117 | 21,000 | 42 | |
IL-33 | 1.06 | 10,000 | 18 | |
IL-36 beta | 0.250 | 2,160 | 15 | |
TNF-alpha | 0.551 | 4,920 | 12 | |
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Trademarks and registered trademarks are the property of their respective companies.
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894