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Format
96-well strip plate
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Assay Length
< 1 hour
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Sample Type & Volume Required Per Well
Urine (10 uL)
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Sensitivity
0.07 mg/dL
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Assay Range
0.3 - 20 mg/dL (Urine)
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Specificity
Measures Creatinine levels in urine
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Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
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Interference
Interference observed with other substances.
Control Available
QC69, Parameter Immunoassay Control Set 685 for Creatinine -
Product Summary
The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Urine | Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.67 | 5.28 | 9.75 | 1.55 | 4.92 | 9.25 |
| Standard Deviation | 0.053 | 0.168 | 0.339 | 0.085 | 0.259 | 0.366 |
| CV% | 3.2 | 3.2 | 3.5 | 5.5 | 5.3 | 4 |
Recovery
The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Urine (n=4) | 96 | 92-97 |
Linearity
To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.
Preparation and Storage
Background: Creatinine
Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.
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Alternate Names
Creatinine;
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