0
* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).
For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
| Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
| Clusterin | 64.3 | 483,800 | 20 | |
| Cystatin C | 57.1 | 16,500 | 19 | |
| CXCL10/IP-10 | 0.693 | 3,110 | 25 | |
| Lipocalin-2/NGAL | 63.1 | 36,100 | 27 | |
| Osteopontin (OPN) | 247 | 295,500 | 28 | |
| RBP4 | 124 | 47,400 | 29 | |
| TFF3 | 11.5 | 11,000 | 30 | |
| TIM-1/KIM-1/HAVCR | 16.3 | 116,000 | 26 | |
| *A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. | ||||
| Analyte | Serum | EDTA Plasma | Heparin Plasma | Urine |
| Clusterin | 1:4,000 | 1:4,000 | 1:4,000 | 1:10 |
| Cystatin C | 1:4,000 | 1:4,000 | 1:4,000 | 1:10 |
| CXCL10/IP-10 | 1:10 | 1:10 | 1:10 | 1:10 |
| Lipocalin-2/NGAL | 1:10 | 1:10 | 1:10 | 1:10 |
| Osteopontin (OPN) | 1:10 | 1:10 | 1:10 | 1:10 |
| RBP4 | 1:4,000 | 1:4,000 | 1:4,000 | 1:10 |
| TFF3 | 1:10 | 1:10 | 1:10 | 1:10 |
| TIM-1/KIM-1/HAVCR | 1:10 | 1:10 | 1:10 | 1:10 |
Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100, Luminex 200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
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