DuoSet Ancillary Kits provide a comprehensive collection of reagents and plates to perform five 96-well assays. DuoSet Ancillary Kit 1 contains Reagent Diluent 1. Each analyte presents varying requirements for optimal assay performance. Please consult the product datasheet for each DuoSet ELISA Development System to determine the required Reagent Diluent formulation.

• Complete kit of supplemental supplies provides an economical way to assay five 96-well ELISA plates.
• For use with DuoSets requiring the following Reagent Diluent formulation: 1.4% delipidized bovine serum, 0.05% Tween® 20 in PBS, pH 7.2-7.4, 0.2 micron filtered.
• Pre-made buffers reduce preparation time and ensure optimal results.

• Clear Polystyrene Microplates (five 12x8-well strip microplates)
• ELISA Plate Sealers (24 plate sealers)
• Color Reagent A (1X, three 12.5 mL vials)
• Color Reagent B (1X, three 12.5 mL vials)
• Stop Solution (1X, three 11 mL vials)
• 20X PBS
• ELISA Plate-coating Buffer (1X, one 60 mL vial)
• Reagent Diluent Concentrate 1 (one 21 mL vial of a buffered protein base)
• Wash Buffer (25X, seven 21 mL vials)
• Reagent Diluent Concentrate 3 (5X, two 21 mL vials of a 25% Tween 20 solution in PBS).
  For Use as the blocking buffer.