Despite the well-characterized factors and protocols used to differentiate mesenchymal stem/stromal cells (MSCs) into chondrocytes, differentiation efficiencies can vary depending on the quality of the MSC starting population and the reagents used to expand and differentiate MSCs.
The term ‘mesenchymal stromal cells’ is commonly used to describe a heterogeneous population of cultured cells that are adherent to plastic, have a distinct morphology, and express a specific set of marker proteins. Within this heterogeneous population are cells referred to as ‘mesenchymal stem cells.’
Mesenchymal stem cells are multipotent, self-renewing cells that have the ability to differentiate into adipocytes, chondrocytes, and osteoblasts when cultured in vitro.
This media supplement contains defined, high quality factors to drive MSC differentiation into chondrocytes.
MSCs Differentiated into Chondrocytes form Characteristic Cell Pellets. Human MSCs cultured with Human/Mouse/Rat StemXVivo® Chondrogenic Base Media (Catalog # ) and Human/Mouse StemXVivo® Chondrogenic Supplement (Catalog # CCM006) formed a chondrogenic pellet (ball) imaged here at day 21 of culture. |
Detection of Aggrecan in a Human MSC-differentiated Chondrogenic Pellet Section. Human MSCs cultured with Human/Mouse/Rat StemXVivo® Chondrogenic Base Media (Catalog # ) and Human/Mouse StemXVivo® Chondrogenic Supplement (Catalog # CCM006) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Goat Anti-Human Aggrecan Antigen Affinity-purified Polyclonal Antibody (Catalog # ). The cells were stained using a NorthernLights™ 557-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # ) and the nuclei were counterstained with DAPI. |
Detection of Collagen II in a Mouse MSC-differentiated Chondrogenic Pellet Section. Mouse MSCs were cultured for 21 days using the Human/Mouse StemXVivo® Chondrogenic Base Media (Catalog # ) and Human/Mouse StemXVivo® Chondrogenic Supplement (Catalog # CCM006) and the resulting chondrogenic pellet was cryosectioned. Chondrocyte differentiation was verified using a Sheep Anti-Mouse Collagen II Antigen Affinity-purified Polyclonal Antibody (Catalog # ). The cells were stained using a NorthernLights™ 557-conjugated Donkey Anti-Sheep Secondary Antibody (Catalog # ). |
Although mesenchymal stromal cells were once referred to as ‘mesenchymal stem cells’, a change to ‘mesenchymal stromal cells’ was proposed by the International Society for Cellular Therapy in 2006.
The change in nomenclature originates from two important factors:
Data supporting MSC self-renewal and multipotency have been obtained using in vitro conditions, which does not adequately reflect the in vivo environment. The lack of in vivo data has led some researchers to question the validity of the term ‘mesenchymal stem cell’ providing further support for the use of ‘mesenchymal stromal cells’ to describe MSCs. While ‘mesenchymal stromal cells’ may be the more scientifically accurate term for MSCs, the two terms are often used interchangeably in the literature. R&D Systems recognizes the use of both mesenchymal stem cells and mesenchymal stromal cells and uses ‘MSC’ to indicate mesenchymal stem/stromal cells to account for both designations.
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