Linkage

Similar to G0876, but produced by Sigma.

Quality

许多来自软体动物的 β-葡萄糖醛酸酶也具有硫酸酯酶活性。
据报道，一些制剂还具有酸性磷酸酶活性。

Unit Definition

在 37°C 和 pH 5.0 下，一个 Sigma 或修正 Fishman 单位每小时可从酚酞葡萄糖苷酸中释放 1.0μg 的酚酞（30 分钟试验）。

硫酸酯酶单位定义：在 pH 5.0 和 37°C 下，一单位硫酸酯酶每小时可水解 1.0μmol 的 4-硝基苯磷二酚硫酸。

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Application

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.^[]

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin.^[1]

Biochem/physiol Actions

β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin the unconjugated form, making bilirubin suitable for reabsorption.^[][][]

β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail. 
Optimal pH: 
• Glucuronidase activity: 4.5 to 5.0 
• Sulfatase activity: ~6.2
Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone

在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.