刀豆凝集素A(Con A) Concanavalin A lectin (Con A) >, 95% 100mg

溶解性	Soluble in water(slightly hazy), and PBS (5 mg/mL, slightly hazy).
存贮条件	储存温度-20°C
密度	否
荧光	否
IC50	否
pK Values	否

溶解性

Soluble in water(slightly hazy), and PBS (5 mg/mL, slightly hazy).

存贮条件

储存温度-20°C

产品介绍	自刀豆和豌豆等籽粒中提取而得的一种植物凝集素(1ectin)。为四聚体球蛋白，分子量102000。每个亚基含237个氨基酸残基，分子量25500，结合一个Ca2+和一个Mn2+，含一个糖结合部位。能与α-甘露糖、α-葡萄糖(细胞膜糖蛋白上)专一地结合，结合位点要求是α-D-吡喃甘露糖或α-D吡喃葡萄糖六元环中的C-3/C-4和C-6未被取代羟基。能与以α-1，2糖苷键连接的甘露二糖和甘露三糖有最大的亲和力。 Concanavalin A is a plant lectin that is a T cell mitogen. It induces apoptosis in human fibroblasts. Concanavalin A (Con A) lectin is isolated from Jack beans (Canavalia ensiformis) and purified by affinity chromatography. The molecular weight of the lectin is 104 kDa. It has broad applicability and is the most widely used lectin within molecular biology research. Con A binds specifically to α-Mannose, α-Galactose structures found in sugars, glycoproteins and glycolipids (2). The lectin has been utilized in hormone receptor studies, mitogenic assays and for characterizing normal and malignant cells (cancer cells are readily aggregated by Con A while normal cells are not). Con A can also initiate cell division (mitogenesis) principally acting on T-lymphocytes (3). Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures. Polyacrylamide gel electrophoresis in SDS of Con A from Jack beans yields three major bands corresponding to molecular weights 27, 13 and 10 kDa. One minor band is visible at 18 kDa. (4). Con A is supplied as a white lyophilized powder from a buffer containing 0.5 mM MnCl2, 0.5 mM CaCl2, no preservatives are added. The identity is determined by SDS-PAGE (Figure 1) and the protein content by spectrophotometry (Figure 2). The lectin is available in vials containing 250 mg or 100 mg lyophilized powder and the product is to be used for laboratory work only.
用途	它为促细胞有丝分裂素(mito-gen)，主要对T淋巴细胞有激发作用，凝集红细胞和动物精子，抑制肿瘤细胞运动，以及延长异源移植存活时间等作用。一种重要的生化和免疫研究试剂。 Appearance: White lyophilized powder Source: Jack beans Molecular weight: 104 kDa Sugar specificity: a-Man, a-Glc Activity: Con A reacts with a number of bacterial and animal cells and can distinguish between certain normal and tumour cells. It can initiate cell division (mitogenesis). 0,5-10 μg/ml is required to visibly agglutinate neuraminidase treated erythrocytes. Microorganisms: < 100 CFU/g Protein content: > 90 %, OD280nm (ε 1mg/ml = 1.14), > 95%, essentially salt free
生化机理	Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
包装	C100881-1g,C100881-25mg,C100881-100mg,C100881-250mg in glass bottle

产品介绍

自刀豆和豌豆等籽粒中提取而得的一种植物凝集素(1ectin)。为四聚体球蛋白，分子量102000。每个亚基含237个氨基酸残基，分子量25500，结合一个Ca2+和一个Mn2+，含一个糖结合部位。能与α-甘露糖、α-葡萄糖(细胞膜糖蛋白上)专一地结合，结合位点要求是α-D-吡喃甘露糖或α-D吡喃葡萄糖六元环中的C-3/C-4和C-6未被取代羟基。能与以α-1，2糖苷键连接的甘露二糖和甘露三糖有最大的亲和力。 Concanavalin A is a plant lectin that is a T cell mitogen. It induces apoptosis in human fibroblasts. Concanavalin A (Con A) lectin is isolated from Jack beans (Canavalia ensiformis) and purified by affinity chromatography. The molecular weight of the lectin is 104 kDa. It has broad applicability and is the most widely used lectin within molecular biology research. Con A binds specifically to α-Mannose, α-Galactose structures found in sugars, glycoproteins and glycolipids (2). The lectin has been utilized in hormone receptor studies, mitogenic assays and for characterizing normal and malignant cells (cancer cells are readily aggregated by Con A while normal cells are not). Con A can also initiate cell division (mitogenesis) principally acting on T-lymphocytes (3). Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures. Polyacrylamide gel electrophoresis in SDS of Con A from Jack beans yields three major bands corresponding to molecular weights 27, 13 and 10 kDa. One minor band is visible at 18 kDa. (4). Con A is supplied as a white lyophilized powder from a buffer containing 0.5 mM MnCl2, 0.5 mM CaCl2, no preservatives are added. The identity is determined by SDS-PAGE (Figure 1) and the protein content by spectrophotometry (Figure 2). The lectin is available in vials containing 250 mg or 100 mg lyophilized powder and the product is to be used for laboratory work only.

用途

它为促细胞有丝分裂素(mito-gen)，主要对T淋巴细胞有激发作用，凝集红细胞和动物精子，抑制肿瘤细胞运动，以及延长异源移植存活时间等作用。一种重要的生化和免疫研究试剂。 Appearance: White lyophilized powder Source: Jack beans Molecular weight: 104 kDa Sugar specificity: a-Man, a-Glc Activity: Con A reacts with a number of bacterial and animal cells and can distinguish between certain normal and tumour cells. It can initiate cell division (mitogenesis). 0,5-10 μg/ml is required to visibly agglutinate neuraminidase treated erythrocytes. Microorganisms: < 100 CFU/g Protein content: > 90 %, OD280nm (ε 1mg/ml = 1.14), > 95%, essentially salt free

生化机理

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

包装

C100881-1g,C100881-25mg,C100881-100mg,C100881-250mg in glass bottle

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

刀豆凝集素A(Con A) Concanavalin A lectin (Con A) >, 95% 100mg

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