Hot Start Taq Master Mix, 2X is a ready-to-use reaction cocktail that contains all required components, except primers template DNA, for routine PCR amplification of DNA fragments up to 4 kb. The included Taq DNA polymerase is inactivated with proprietary monoclonal antibodies. Incubation at ≥ 94°C during PCR thermal cycling irreversibly denatures the antibody releases full Taq DNA polymerase activity.
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