Overview

• Transfer to development in less than 90 minutes
• Rapid Transfer Buffer transfers proteins from gel to membrane in 10 - 20 minutes
• RapidBlock™ efficiently blocks the membrane in 5 minutes
• Rapid Blot Secondary HRP Antibody is conincubated with primary antibody to save time
• Use Rapid Wash Buffer for a single, 15 minute wash step
• Obtain results fast

Detailed Description

AMRESCO’s Rapid Western Blotting Kits streamline each step of Western blotting, enabling the entire procedure from the transfer step through development to be completed in less that an hour a half. These versatile kits include all reagents except for user-supplied primary antibodies are designed for use with standard transfer equipment. All reagents are protein-free formulations to minimize cross-reactivity enhance antigen availability. Rapid Western Blotting Kits are offered with anti-mouse, anti-rabbit, anti-goat, anti-rat secondary antibodies that are optimized to generate blots with low backgrounds reduced blotches. The wet transfer semi-dry versions of the kits, which differ only in the amount of transfer buffer included, are packaged with sufficient reagents to perform fifteen 10 cm x 10 cm blots. Rapid Western Blotting Kits are compatible with both PVDF nitrocellulose membranes with staining with phospho-specific antibodies. 

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