Recombinant M-MLV Reverse Transcriptase purified from E. coli expressing the pol gene of Moloney Murine Leukemia Virus.
RT-PCR of GAPDH from varying amounts of HeLa cell total RNA. First-strand synthesis was carried out with log-fold dilutions of HeLa cell total RNA (1 µg – 1 pg), no RNA (NTC) oligo dT(20) primer according to the recommended protocol. Each first-strand reaction (1/10th) was used as template for PCR amplification of a 555-bp fragment from the GAPDH gene using Hot Start PCR-to-Gel Master Mix , 2X. PCR was carried out in a 20 µL reaction a standard Hot Start PCR protocol was followed. Each PCR product (5 µL) was separated on a 1.2% agarose, 0.5X TBE, 0.25 µg/mL ethidium bromide gel.
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894