·   Eliminates ethidium bromide hazards

·   Increases ready mix stability room temperature storage

·   Streamlines PCR sample handling processing

Lyophilized Ready PCR Mix, 2X, offers the same conveniences of our Ready PCR Mix, 2X, but in a lyophilized form, allowing greater stability. The lyophilized formulation allows room temperature storage until dissolution with water. All components for assembly performance of PCR reactions as well as loading visualization of PCR products on agarose gels are included. The user supplies high purity grade water, primer template DNA.

Lyophilized Ready PCR Mix is supplied as a 2X powder of reaction buffer, AMRESCO’s Extender™ Taq polymerase blend, dNTPs, electrophoresis tracking dye a non-mutagenic EZ-Vision® visualization dye.  The powder can be stored at room temperature prior to dissolving in water. Once dissolved, the reaction mix must be stored frozen. Similar to AMRESCO’s Ready PCR Mix, when amplification is complete, the PCR reaction can be directly loaded separated on an agarose gel using the magenta-colored tracking dye (migrating at approximately 10bp on a 1% gel) to monitor the migration. After electrophoresis, the PCR products are immediately visualized with standard UV illumination without additional post-run staining destaining steps. AMRESCO’s Lyophilized Extender™ PCR-to-Gel Master Mix, 2X contains the same formulation as the Lyophilized Ready PCR Mix, 2X without the fluorescent DNA Dye.

Available in 1 2 vial pack sizes. One vial is enough to run 50 reactions based on a 50 microliter reaction size.
Also available in a ready-to-use master mix solution. See Ready PCR Mix, 2X.

Click on the Features tab to learn more about Lyophilized Ready PCR Mix, 2X