Extender™ PCR-to-Gel Master Mix, 2X offers a single-step procedure for performing PCR reactions.

• Saves time
• Streamlines PCR sample handling processing
• Includes all reagents expect fluorescent DNA dye, DNA template, primer DNA

Extender™ PCR-to-Gel Master Mix, 2X offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer. The master mix includes Extender™ Taq Polymerase, dNTPs, MgCl2, tracking dyes. The user supplies primer template DNA. Aliquot of the PCR reaction can be directly loaded onto an agarose gel.

Kit includes 2 x 1.25 ml tubes of 2X reaction mix. Combine primers, DNA template, nuclease-free water, Extender™ PCR-to-Gel Master Mix, 2X in a PCR tube. After product amplication, directly load the PCR reactions to a gel separate. Once electrophoresis is complete, visualize the DNA product using a standard UV illuminator.

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