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Qiagen 凯杰 Biosharp Omega

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EXTENDER™ DNA POLYMERASE BLEND, 500U/DNA聚合酶混合物

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号:N638-KIT

牌:Amresco

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Overview

 

  • Superior yields purity
  • Ideal for amplification of long templates
  • Excellent for hard-to-copy templates
  • Amplified products include a mixture of blunt ends 3'dA overhangs
  • Wide tolerance for Mg+2, salt concentration, pH template contaminants

 

 

Detailed Description

Extender™ Taq DNA Polymerase is a high fidelity, thermostable enzyme blend optimized to generate high product yields purity. This polymerase blend includes 3' to 5’ proofreading activity for accurate template replication. It is recommended for amplification of critical templates containing GC-rich regions, palindromes, multiple repeats. Extender™ Polymerase exhibits wide tolerance for Mg+2  salt concentrations, making it excellent for multiplex amplifications. A mixture of blunt end 3'dA overhang amplification products are generated. Amplification of DNA products up to 20 kb can be achieved with the optimized buffers included in the kit. Kit includes 1 tube of Extender™ Taq DNA Polymerase Blend, 500 U (5 U/µL), 1 tube of Extender™ Taq DNA Polymerase Buffer A, 10X (without MgCl2), 1 tube of Extender™ Taq DNA Polymerase Buffer S, 10X (with MgCl2), 1 tube of Extender Polymerase MgCl2, 50 mM. Simply, choose the appropriate buffer magnesium concentration.  Combine the buffer, magnesium, dNTPs, primers, template DNA, 2 U of Extender™ DNA Polymerase Blend.  Amplify the DNA with an optimized PCR cycle.

 

Click on the Features tab to learn more about Extender™ DNA Polymerase Blend, 500U

 

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