See All Kinase Enzyme Systems
Recombinant full-length human CDK3 Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1is a member of the cyclin-dependent protein kinase family promotes entry S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis (1). The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: CDK3/CyclinE1, 10μg (Human, recombinant full-length). MW: ~60kDa (CDK3) ~73kDa (CyclinE1).
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Substrate: Native Histone H1 Protein; purified from calf thymus tissues.
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Other: Reaction Buffer, DTT.
CDK3/CyclinE1 NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.