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Full-length recombinant human CK1α1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1α1 is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes. CK1 can regulate Smo cell surface accumulation activity in response to hedgehog. CK1 phosphorylates Smo at several sites, phosphorylation-deficient forms of Smo fail to accumulate on the cell surface are unable to transduce the hedgehog signal (1). CK1α1 dynamically associates with the CBM complex on T cell receptor engagement to participate in cytokine production lymphocyte proliferation. CK1α1 can form complexes with MDM2, which then regulates the stability of p53 E2F-1 transcription factors (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Human Kinase Enzyme Systems Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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