See All Kinase Enzyme Systems
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility transformation. These serine/threonine kinases are activated by are effectors of small GTPases, CDC42 RAC. PAK1 is one of the Group I PAKs, which also include PAK2 PAK3 (1). PAK1 is a key regulator of the actin cytoskeleton, adhesion cell motility. Inactive dimeric PAK1 is mainly cytosolic interaction with the activators Cdc42-GTP Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: PAK1/CDC42, 10μg (Human, recombinant full-length). MW: ~96kDa (PAK1).
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Substrate: PAKtide (RRRLSFAEPG).
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Other: Reaction Buffer, DTT, GTP Solution.
PAK1/CDC42 NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.