See All Kinase Enzyme Systems 
Recombinant human MLK2 (1–446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 MKK4/SEK1. MLK2 itself can be phoshorylated activated by JNK kinases. MLK2 is also known as mitogen-activated protein kinase kinase kinase 10 (MAP3K10). MLK2 functions preferentially on the JNK signaling pathway, which is involved in nerve growth factor (NGF)-induced neuronal apoptosis. MLK2 was isolated as a cDNA fragment from MKN28 gastric cancer cell library using degenerate PCR (1). MLK2 is highly expressed in brain, skeletal muscle testis (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: MLK2, 10μg (Human, recombinant; amino acids 1–446). MW: ~76kDa.
• Substrate: Native Swine Myelin Basic Protein (MBP).
• Other: Reaction Buffer, DTT.

MLK2 NCBI Database Entry.

Human Kinase Enzyme Systems Applications.

特点 - 优点

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.