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Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SLK LOSK is a Ste20-like protein kinase that associates with microtubule centrosome. Inhibition of SLK activity by dominant-negative mutant RNAi leads to unfocused microtubule arrangement, indicating that SLK is needed for microtubule organization proper localization of Golgi complex (1). Expression of v-Src can downregulate SLK activity through the involvement of CK2, which can directly phosphorylate inhibit SLK activity, whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity (2). CK2 SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Human Kinase Enzyme Systems Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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