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Recombinant human PASK (981–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress. PASK is involved in sensing environmental changes in light intensity, oxygen concentration redox potential. Through interaction with IRS-1, PASK has been proposed as a counter-regulatory mechanism in insulin cytokine signaling (1). PASK can phosphorylate inactivate glycogen synthase in vitro. Efficient phosphorylation requires residues 444 to 955 of PASK between the PAS catalytic kinase domain, this interaction is inhibited by the PAS domain.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Human Kinase Enzyme Systems Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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