See All Kinase Enzyme Systems
Recombinant human PASK (981–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress. PASK is involved in sensing environmental changes in light intensity, oxygen concentration redox potential. Through interaction with IRS-1, PASK has been proposed as a counter-regulatory mechanism in insulin cytokine signaling (1). PASK can phosphorylate inactivate glycogen synthase in vitro. Efficient phosphorylation requires residues 444 to 955 of PASK between the PAS catalytic kinase domain, this interaction is inhibited by the PAS domain.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: PASK, 10μg (Human, recombinant; amino acids 981–end). MW: ~66kDa.
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Substrate: ZIPtide (KKLNRTLSFAEPG).
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Other: Reaction Buffer, DTT.
PASK NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.