See All Kinase Enzyme Systems 
Recombinant full-length human p38β was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38β is a member of the p38 MAP kinase family is activated by both proinflammatory cytokines environmental stress (1). The p38β is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase (2). Alternatively spliced transcript variants encoding the same protein have been observed.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: p38β, 10μg (Human, recombinant full-length). MW: ~71kDa.
• Substrate: p38 substrate (IPTTPITTTYFFFKKK).
• Other: Reaction Buffer, DTT.

p38β NCBI Database Entry.

Human Kinase Enzyme Systems Applications.

特点 - 优点

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.