See All Kinase Enzyme Systems
Recombinant human DAPK1 (1–363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by γ-interferon. Activation of DAPK occurs via dephosphorylation of Ser-308 subsequent association of calcium/calmodulin (1). DAPK is rapidly dephosphorylated in response to tumor necrosis factor ceramide then subsequently degraded via proteasome activity. The decline in DAPK expression is paralleled with increased caspase activity cell apoptosis. Studies suggest that the apoptosis regulatory activities mediated by DAPK are controlled both by phosphorylation status protein stability (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: DAPK1, 10μg (Human, recombinant; amino acids 1–363). MW: ~71kDa.
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Substrate: Native Swine Myelin Basic Protein (MBP).
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Other: Reaction Buffer, DTT, Ca2+/Calmodulin Solution.
DAPK1 NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.