See All Kinase Enzyme Systems
Recombinant human proteins TAK1 (1–303) TAB1 (437–end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ morphogenetic protein (BMP) (1). In response to IL-1, TAK1 forms a kinase complex with TAB1, this complex is required for the activation of nuclear factor kappa B (NF-κB). TAK1 also can activate MAPK8/JNK MAP2K4/MKK4 thus play a role in the cell response to environmental stress. TAK1 is essential for thymocyte development activation, deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 CD8 (2). Thymocytes lacking TAK1 fail to activate NF-κB JNK are prone to apoptosis upon stimulation.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
-
Kinase: TAK1-TAB1, 10μg (Human, recombinant; TAK1 (1–303) TAB1 (437–end)). MW: ~74kDa.
-
Substrate: Native Swine Myelin Basic Protein (MBP).
-
Other: Reaction Buffer, DTT.
TAK1 NCBI Database Entry.
TAB1 NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
-
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
-
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
-
Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.