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Recombinant full-length human p38δ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38δ (SAPK4) is a member of the p38 MAPK family is activated by chemical environmental stresses as well as by proinflammatory cytokines. p38δ has a TGY dual phosphorylation motif is activated in response to cellular stresses proinflammatory cytokines (1). MAP kinase kinases 3 6 can phosphorylate activate this kinase. Transcription factor ATF2 microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: p38δ, 10μg (Human, recombinant full-length). MW: ~71kDa.
• Substrate: p38 substrate (IPTTPITTTYFFFKKK).
• Other: Reaction Buffer, DTT.

p38δ NCBI Database Entry.

Human Kinase Enzyme Systems Applications.

特点 - 优点

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.