See All Kinase Enzyme Systems
Recombinant human NIK (325–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 stimulates NF-κB activity. NIK shares sequence similarity with several other MAPKK kinases participates in NF-κB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family to the interleukin-1 type-I receptor (1). NIK is expressed in primary human cells inflamed rheumatoid arthritis tissue plays a selective role in signaling by the lymphotoxinβ receptor (2). NIK is a therapeutic target in the immune bone-destructive components of inflammatory arthritis.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
-
Kinase: NIK, 10μg (Human, recombinant; amino acids 325–end). MW: ~108kDa.
-
Substrate: Native Swine Myelin Basic Protein (MBP).
-
Other: Reaction Buffer, DTT.
NIK NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
-
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
-
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
-
Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.