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Recombinant human MYO3β (1–326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3β is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain. MYO3β contains an N-terminal kinase domain, followed by motor, neck tail domains. The MYO3β gene generates a variety of splice variants that contain 1 2 calmodulin-binding (IQ) motifs in the neck domain 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7kb MYO3β transcript in the human retina but not in the RPE cell line (1). The MYO3β gene transcript is also detected in the kidney, intestine testis. The MYO3β gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: MYO3β, 10μg (Human, recombinant; amino acids 1–326). MW: ~63kDa.
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Substrate: Native Swine Myelin Basic Protein (MBP).
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Other: Reaction Buffer, DTT.
MYO3β NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.