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Recombinant human MYO3β (1–326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3β is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain. MYO3β contains an N-terminal kinase domain, followed by motor, neck tail domains. The MYO3β gene generates a variety of splice variants that contain 1 2 calmodulin-binding (IQ) motifs in the neck domain 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7kb MYO3β transcript in the human retina but not in the RPE cell line (1). The MYO3β gene transcript is also detected in the kidney, intestine testis. The MYO3β gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Human Kinase Enzyme Systems Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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