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Full-length recombinant human IKKα was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKKα is a serine/threonine protein kinase that phosphorylates the I-κB protein, which is an inhibitor of the transcription factor NF-κB complex. Phosphorylation of I-κB protein triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-κB complex (1). IKKα is an essential regulator of NF-κB-dependent gene expression through control of promoter-associated histone phosphorylation after cytokine exposure (2). IKKα is a critical component of the cytoplasmic transductional-transcriptional processor leading to induction of IFNα production. IKKα is also involved in the epidermis, where it antagonizes mitogenic angiogenic signals represses tumor progression metastases.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Human Kinase Enzyme Systems Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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