See All Kinase Enzyme Systems 
Recombinant human FLT3 (571–993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT3 is a receptor tyrosine kinase that has been shown to play a role in proliferation survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors (1). FLT3 consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region a cytoplasmic kinase domain split two parts by a kinase- domain. FLT3 is the most frequently mutated gene in cases of acute myelogenous leukemia (AML). About 30% to 35% of patients have either internal tandem duplications (ITDs) in the juxtamembrane domain mutations in the activating loop of FLT3 (2). The consequence of either FLT3-ITD activating loop mutations is the constitutive activation of the tyrosine kinase activity.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: FLT3, 10μg (Human, recombinant; amino acids 571–993). MW: ~73kDa.
• Substrate: Native Swine Myelin Basic Protein (MBP).
• Other: Reaction Buffer, DTT.

FLT3 NCBI Database Entry.

Human Kinase Enzyme Systems Applications.

特点 - 优点

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.