See All Kinase Enzyme Systems
Recombinant full-length human RSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK1 is a member of the RSK (ribosomal S6 kinase) family that consists of growth factor-regulated serine/threonine kinases. RSK1 contains 2 nonidentical kinase catalytic domains phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. RSK1 encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver adipose tissue (1). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade the stimulation of cell proliferation differentiation (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: RSK1, 10μg (Human, recombinant full-length). MW: ~108kDa.
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Substrate: S6K Substrate (KRRRLASLR); derived from human 40S ribosomal protein S6 (amino acids 230–238).
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Other: Reaction Buffer, DTT.
RSK1 NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.