See All Kinase Enzyme Systems
Recombinant full-length human PKC epsilon (PKCε) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKCε is a member of the protein kinase C (PKC) family of serine- threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKCε is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia (1), heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages may control anxiety-like behavior (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
-
Kinase: PKCε, 10μg (Human, recombinant full-length). MW: ~110kDa.
-
Substrate: PKCtide (ERMRPRKRQGSVRRRV); derived from protein kinase C epsilon (amino acids 149–164).
-
Other: Reaction Buffer, DTT, Lipid Solution.
PKCε NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
-
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
-
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
-
Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.