See All Kinase Enzyme Systems
Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT3 Protein Kinase B γ (PKBγ) is a serine/threonine kinase that is a member of the AKT family. AKT3 is activated in cells exposed to diverse stimuli such as hormones, growth factors extracellular matrix components (1). AKT3 phosphorylates regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis proliferation. Recent evidence indicates that AKT3 is frequently overexpressed in many types of human cancers including breast prostate (2).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: AKT3, 10μg (Human, recombinant full-length). MW: ~85kDa.
• Substrate: Akt (SGK) substrate peptide (RPRAATF); derived from the N-terminus of GSK3.
• Other: Reaction Buffer, DTT.

AKT3 NCBI Database Entry.

Human Kinase Enzyme Systems Applications.

特点 - 优点

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
• Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
• Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

注意事项

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.