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Recombinant human TGFβR2 (190–end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TGFβR2 is a member of the TGFβ receptor subfamily is a ser/thr protein kinase. TGFβR2-induced protein phosphorylation plays a key role in signal transduction that leads to mitogenic responses. The TGFβR2 receptor transmits signals from the cell surface to the nucleus provides instructions for making transforming growth factor (TGF)-beta type II receptor. Mutations in TGFβR2 gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome the development of various types of tumors.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
Human Kinase Enzyme Systems Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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