See All Kinase Enzyme Systems
Recombinant full-length human PKC delta (PKCδ) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79kDa protein kinase that shows strict dependence on the presence of phospholipids but shows no activation by Ca2+. Phosphatidylinositol is the most potent activator of PKCδ. Northern blot analysis indicated that PKCδ is widely distributed in almost all tissues is a major isoform of PKC expressed in hemopoietic cells. PKCδ is involved in fundamental cellular functions regulated by diacylglycerols mimicked by phorbol esters.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
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Kinase: PKCδ, 10μg (Human, recombinant full-length). MW: ~104kDa.
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Substrate: CREBtide (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109–121).
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Other: Reaction Buffer, DTT, Lipid solution.
PKCδ NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
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Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
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Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
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Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.