See All Kinase Enzyme Systems
Recombinant full-length human PAK4 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. PAK4 is a recently identified member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility transformation. These serine/threonine kinases are activated by are effectors of small GTPases, cdc 42 Rac. PAK4 belongs to the Group II PAKs that also includes PAK5 PAK6. PAK4 has been shown to regulate cell morphology cytoskeletal organization in mammalian cells.
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
-
Kinase: PAK4, 10μg (Human, recombinant full-length). MW: ~90kDa.
-
Substrate: Modified AKT Substrate II peptide (modified-CKRPRAASFAE); based on the N-terminus of GSK3.
-
Other: Reaction Buffer, DTT.
PAK4 NCBI Database Entry.
Human Kinase Enzyme Systems Applications.
特点 - 优点
-
Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up running in no time.
-
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT supplemental reagents as needed.
-
Obtain Reliable Results: The broad dynamic range, the ease of use better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.
注意事项
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.