The GoTaq® qPCR Master Mix is optimized for fast reproducible quantitative PCR assays using a double-stranded DNA-binding dye. The master mix contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum PCR efficiency greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR® Green I.

The master mix is provided as a ready-to-use, stabilized 2X formulation contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoTaq® 2-Step RT-qPCR System facilitates detection relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System GoTaq® qPCR Master Mix.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

The GoTaq® 1-Step RT-qPCR System enables detection relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor additives to enhance single-step reactions.

特点 - 优点

• Achieve Superior Performance: Sensitive detection for earlier quantitation of low- high-copy-number targets over a broad dynamic range.
• Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
• Use a Versatile Product: Compatible with both fast standard cycling methods on most real-time PCR instruments.
• Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated high-throughput detection.