The ReliaPrep™ 96 gDNA Miniprep HT System provides a simple reliable method for the rapid isolation of gDNA in a multiwell format. gDNA may be purified from blood Oragene®•Discover sample collection devices. The purified gDNA can be used directly in PCR assays, microarrays next-generation sequencing applications. The use of paramagnetic particles for DNA capture eliminates the need for centrifugation vacuum manifolds, making the system suitable for full automation. In addition, the system does not require an organic solvent, making it safe convenient. DNA yields of up to 12µg are expected from input blood volumes of 350µl, depending on the WBC of the sample. Saliva samples can have variable amounts of gDNA, up to 18µg more of DNA may be recovered from a 700µl Oragene® collection device sample.

特点 - 优点

• Improve Productivity: Walkaway automation of genomic DNA extraction.
• Eliminate Sample Rework: Robust, precipitation-free protocol, no chance of "lost pellets".
• Simplify Workflow: High yields of pure DNA from pristine challenged hemolysed samples.
• Reduce Time to Results: Pure gDNA ready for demanding applications; samples in solution; no resuspension required.

应用

Purified DNA is suitable for common assays including genotyping, PCR, qPCR sequencing.